Procathepsin S, human recombinant

Catalog number

7525-10

Size

10 μg

Price

375.00 EUR

Supplier

Biovision

Order

Details

Synonyms

CTSS

Alternates names

CTSS

Taglines

A lysosomal cysteine protease participating in the degradation of antigenic proteins to peptides

Biomolecule/Target

Procathepsin S

NCBI Gene ID #.

1520

NCBI Gene Symbol

CTSS

Gene Source

Human

Accession #

P25774

Recombinant

Yes

Source

E. coli

Purity

N/A

Endotoxin Level

N/A

Activity (Specifications/test method)

N/A

Biological activity

Biovision’s Procathepsin S can be auto-catalytically activated to mature Cathepsin S (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at room temperature (RT). The enzyme is fully activated within 2-3 hrs. of incubation at RT. After activation mature Cathepsin S is highly auto-proteolytic and care must be taken to avoid self-proteolysis.

Results

>1000 mU/mg (1 U = Digestion of 1 µmole/min) of Ac-VVR-AFC substrate (K144-100) after full activation.

Molecular Weight

38.1 kDa (17-331 aa + N-terminal poly-his tag)

Storage Temp.

-20°C

Shipping

Gel Pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized

Physical form description

Lyophilized from proprietary buffer.

Reconstitution Instructions

Reconstitute in 25 mM Na2HPO4, 500 mM NaCl (pH 7.0) to 0.5-1 mg/ml

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants