Procathepsin S, human recombinant

Catalog number
7525-500
Name
Procathepsin S, human recombinant
Size
500 μg
Price
7100.00 EUR
Supplier

Details

Synonyms
CTSS
Alternates names
CTSS
Taglines
A lysosomal cysteine protease participating in the degradation of antigenic proteins to peptides
Biomolecule/Target
Procathepsin S
NCBI Gene ID #.
1520
NCBI Gene Symbol
CTSS
Gene Source
Human
Accession #
P25774
Recombinant
Yes
Source
E. coli
Purity
N/A
Endotoxin Level
N/A
Activity (Specifications/test method)
N/A
Biological activity
Biovision’s Procathepsin S can be auto-catalytically activated to mature Cathepsin S (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at room temperature (RT). The enzyme is fully activated within 2-3 hrs. of incubation at RT. After activation mature Cathepsin S is highly auto-proteolytic and care must be taken to avoid self-proteolysis.
Results
>1000 mU/mg (1 U = Digestion of 1 µmole/min) of Ac-VVR-AFC substrate (K144-100) after full activation.
Molecular Weight
38.1 kDa (17-331 aa + N-terminal poly-his tag)
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from proprietary buffer.
Reconstitution Instructions
Reconstitute in 25 mM Na2HPO4, 500 mM NaCl (pH 7.0) to 0.5-1 mg/ml
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants