G6PD, Human Recombinant
Catalog number
7532-100
Name
G6PD, Human Recombinant
Size
100 μg
Price
475.00 EUR
Supplier
Details
Synonyms
Glucose-6-phosphate 1-dehydrogenase, Zwf.
Alternates names
Glucose-6-phosphate 1-dehydrogenase, Zwf.
Taglines
Rate-limiting enzyme of the pentose phosphate pathway
Biomolecule/Target
G6PD
NCBI Gene ID #.
2539
NCBI Gene Symbol
G6PD
Gene Source
Human
Accession #
P11413
Recombinant
Yes
Source
Hi-5 cells, Baculovirus system
Purity
N/A
Endotoxin Level
N/A
Activity (Specifications/test method)
N/A
Biological activity
Specific activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 µmole D-glucose-6-phosphate to 6-phospho-Dgluconate per min in the presence of beta-NADP at pH 7.4 at 25ºC.
Results
Specific activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 µmole D-glucose-6-phosphate to 6-phospho-Dgluconate per min in the presence of beta-NADP at pH 7.4 at 25ºC.
Molecular Weight
61.4 kDa (515 aa, 1-515 aa)
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
6 months
Concentration
0.5 mg/ml
Appearance
Liquid
Physical form description
0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 mM PMSF, 2 mM EDTA, 2 mM DTT, 200 mM NaCl.
Reconstitution Instructions
N/A
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
