Biotin-NHS for cell activation

Catalog number
2347-1000
Name
Biotin-NHS for cell activation
Size
1 g
Price
469.00 EUR
Supplier

Details

Description
Biotin-NHS is a useful reagent for biotinylation of primary amines under mild conditions (pH~6.5-8.5). Some examples of primary amines for biotinylation include lysines on the surface of proteins, antibodies, etc.
Peptide sequence
N/A
CAS number
35013-72-0
Molecular weight
341.38
Category
Biochemicals
Other name
Biotin-N-hydroxysuccinimide ester; (+)-Biotin N-succinimidyl ester, d-Biotin NHS ester, BNHS, Biotinyl-N-hydroxy-succinimide, N-Hydroxysuccinimidobiotin
Molecular formula
C₁₄H₁₉N₃O₅S
Physical appearance
White to Off-white Solid
Supplied with
N/A
Is this a salt?
No
Is it cell-permeable?
No
Purification
≥95% by HPLC
Reconstitute instructions
DMF (~ 50 mg/ml)
Storage condition
-20 ̊C
Shipping condition
gel pack
Maximum time for storage
36 months
Storage instructions
Protect from air and moisture
Tissue
cell
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
Additional description
For cells, cell lines and tissues in culture till half confluency.