Human CellExp™ 4-1BB / TNFRSF9, Human recombinant

Catalog number
9227-50
Name
Human CellExp™ 4-1BB / TNFRSF9, Human recombinant
Size
50 µg
Price
713.00 EUR
Supplier

Details

Target
CD137
Background
4-1BB is also known as CD137, tumor necrosis factor receptor superfamily member 9 (TNFRSF9), induced by lymphocyte activation (ILA), is a co-stimulatory molecule of the tumor necrosis factor (TNF) receptor superfamily. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice. CD137 can enhance activation-induced T cell apoptosis when triggered by engagement of the TCR/CD3 complex. In addition, 4-1BB/4-1BBL co-stimulatory pathway has been shown to augment secondary CTL responses to several viruses, and meanwhile augment anti-tumor immunity. 4-1BB thus is a promising candidate for immunotherapy of human cancer. CD137 has been shown to interact with TRAF responses to several viruses, and meanwhile augment anti-tumor immunity. 4-1BB thus is a promising candidate for immunotherapy of human cancer. CD137 has been shown to interact with TRAF
Concentration
N/A
Molecular weight
This protein is fused with a 6× His tag at C-terminus and has a calculated MW of 18.1 kDa. The protein migrates as 28-35 kDa in SDS-PAGE due to glycosylation.
Synonym
TNFRSF9,4-1BB,CD137,CDw137,ILA
Other name
TNFRSF9,4-1BB,CD137,CDw137,ILA
NCBI gene number
3604
NCBI gene
TNFRSF9
Gene source
Human
NCBI number
NP_001552.2
Recombinant
Yes
Source
HEK 293 cells
Purification
≥92%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
< 1 EU/μg
Tested activity
Measured by its binding ability in a functional ELISA
Biological activity
Immobilized Human 4-1BB, His Tag at 100 ng/Ml (100 µl/well),can bind Human 4-1BB Ligand, Fc Tag with a linear of 0.1-2.5 ng/mL.
Reesults
N/A
Binding ability
Immobilized Human 4-1BB, His Tag at 100 ng/Ml (100 µl/well),can bind Human 4-1BB Ligand, Fc Tag with a linear of 0.1-2.5 ng/mL.
Unit
N/A
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants