Human CellExp™ BTLA, Human recombinant

Catalog number
9228-10
Name
Human CellExp™ BTLA, Human recombinant
Size
10 µg
Price
289.00 EUR
Supplier

Details

Target
CD272
Background
B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.
Concentration
N/A
Molecular weight
This protein is fused with a human IgG1 Fc tag at C-terminus and has a calculated MW of 38.7 kDa. The protein migrates as 45-50 kDa in SDS-PAGE due to glycosylation.
Synonym
BTLA, CD272
Other name
BTLA, CD272
NCBI gene number
151888
NCBI gene
BTLA
Gene source
Human
NCBI number
NP_001078826.1
Recombinant
Yes
Source
HEK 293 cells
Purification
≥92%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
< 1 EU/μg
Tested activity
Measured by its binding ability in a functional ELISA
Biological activity
Immobilized Human HVEM, C-Twin Streptavidin Tag at 2 ng/ml (100 µl/well),can bind Human BTLA, Fc Tag with a linear of 0.1-0.5 μg/mL.
Reesults
N/A
Binding ability
Immobilized Human HVEM, C-Twin Streptavidin Tag at 2 ng/ml (100 µl/well),can bind Human BTLA, Fc Tag with a linear of 0.1-0.5 μg/mL.
Unit
N/A
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants