Human CellExp™ CTLA-4/CD152, mouse recombinant

CTLA4

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

N/A

41.1 kDa

CTLA4, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12

CTLA4, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12

12477

CTLA4

Mouse

P09793

Yes

HEK 293 cells

>95%

SDS-PAGE, N/A

< 1 EU/μg

Measured by its binding ability in a functional ELISA.

Immobilized Mouse B7-1 and B7-2, Fc Tag at 0.2 μg/mL (100 μ L/well) can bind Mouse CTLA-4, mouse IgG2a Fc tag with a linear range of 0.1-3 ng/mL and 0.4-3 ng/mL respectively.

N/A

Immobilized Mouse B7-1 and B7-2, Fc Tag at 0.2 μg/mL (100 μ L/well) can bind Mouse CTLA-4, mouse IgG2a Fc tag with a linear range of 0.1-3 ng/mL and 0.4-3 ng/mL respectively.

N/A

-20°C

Gel pack

Lyophilized

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitute in sterile deionized water to a concentration of 50 μg/ml.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus

Recombinants or rec. proteins

recombinants