FractionPREP™ Cell Fractionation Kit

Catalog number
K270-50
Name
FractionPREP™ Cell Fractionation Kit
Size
50 assays
Price
510.00 EUR
Supplier

Details

Kit's description
This FractionPREP™ Cell Fractionation system is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.
Highlights
• Species reactivity: Mammalian • Applications: 1:D or 2:D gel, enzyme activity assays, gel shift assay, and Western blotting.
Kit's benefits
• Simple procedure; takes less than 1 hour • Fast and convenient • The kit is designed for serial sample preparation of four distinct protein fractions including cytosol/particulate/cytoskeleton/nuclear fractions, from one sample.
Category
Cell Fractionation Kit
Kit's other name
none
Contents
• Cytosol Extraction Buffer (CEB) • Membrane Extraction Buffer-A (MEB-A) • Membrane Extraction Buffer-B (MEB-B) • Nuclear Extraction Buffer (NEB) • DTT (1 M) • Protease Inhibitor Cocktail (lyophilized)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell cultures (adherent and suspension), fresh and frozen tissues
Tissue
cell
Description
For cells, cell lines and tissues in culture till half confluency.