LDH-Cytotoxicity Colorimetric Assay Kit

Catalog number
LDH-Cytotoxicity Colorimetric Assay Kit
400 assays
368.00 EUR


Kit's description
Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. The LDH-Cytotoxicity Assay Kit provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or unstable, LDH is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm. The LDH-cytotoxicity assay is sensitive, convenient, and precise, and is applicable to a variety of cytotoxicity studies. Assay takes ~0.5–1 hr.
• Detection method: Absorbance (500 nm) • Sample type: Cell culture (adherent and suspension) • Species reactivity: Mammalian • Applications: This assay provides a convenient and non:radioactive alternative to the [3H]:thymidine and the [51Cr] release assays The assay is based on measurement of activity of lactate dehydrogenase (LDH) which is a stable enzyme normally found in the cytosol of all cells but rapidly releases into the supernatant upon damage of plasma membrane.
Kit's benefits
• Simple one-step procedure; takes less than 1 hour • Fast, sensitive and convenient
Cytotoxicity Assay
Kit's other name
• Catalyst, Lyophilized • Dye Solution
Storage condition
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Samples tested
Cell cultures (adherent and suspension)
Biovision supplies other types of Assays as 1.
Colorimetric assays or detection use UV absorption or enzymatic color reaction.