HAT Activity Fluorometric Assay Kit
Catalog number
K334-100
Name
HAT Activity Fluorometric Assay Kit
Size
100 assays
Price
500.00 EUR
Supplier
Details
Kit's description
Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases. BioVision’s HAT Activity Assay Kit utilizes Acetyl CoA and H3 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl-CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity as low as 6 mU in a variety of samples.
Highlights
• Detection method: Fluorescence (Ex/Em = 535/587 nm) • Applications: : Measurement of HAT activity in Nuclear Extracts : Measurement of HAT activity of purified enzyme preparations
Kit's benefits
• Simple, rapid & convenient • The assay can detect HAT Activity less than 6 mU in a variety of samples
Category
HAT activity
Kit's other name
Histone Acetyltransferase Activity Fluorometric Assay Kit
Contents
• HAT Assay Buffer • Acetyl CoA (Lyophilized) • H3 Peptide (Lyophilized) • Substrate Mix (Lyophilized) • Developer • PicoProbe™ • CoA Standard (Lyophilized) • Positive Control (HeLa Nuclear Extract)
Storage condition
-80°C
Shipping condition
dry ice
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Measurement of HAT activity in Nuclear Extracts • Measurement of HAT activity of purified enzyme preparations
Samples tested
• Nuclear extracts from cells and tissue • Recombinant enzyme
Test
Biovision supplies other types of Assays as 1.