HAT (H4) Activity Fluorometric Assay Kit

Catalog number
K336-100
Name
HAT (H4) Activity Fluorometric Assay Kit
Size
100 assays
Price
500.00 EUR
Supplier

Details

Kit's description
Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases. BioVision’s HAT Activity Assay Kit utilizes Acetyl CoA and H4 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl-CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer and PicoProbeTM to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity equivalent to as low as 1.5 µU of p300 in a variety of samples.
Highlights
• Detection method: Fluorescence (Ex/Em = 535/587 nm) • Applications: Measurement of HAT activity of immunoprecipitated samples : Measurement of HAT activity of purified enzyme preparations : Measurement of HAT activity in Nuclear Extracts.
Kit's benefits
• Simple, rapid & convenient • The assay can detect HAT Activity equivalent to as low as 1.5 µU of p300 in a variety of samples.
Category
HAT activity
Kit's other name
Histone Acetyltransferase (H4) Activity Fluorometric Assay Kit
Contents
• HAT Assay Buffer • Acetyl CoA (Lyophilized) • H4 Peptide (Lyophilized) • Substrate Mix (Lyophilized) • Developer • PicoProbe™ • CoA Standard (Lyophilized) • Positive Control (HeLa Nuclear Extract)
Storage condition
-80°C
Shipping condition
dry ice
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Measurement of HAT activity of immunoprecipitated samples • Measurement of HAT activity of purified enzyme preparations • Measurement of HAT activity in Nuclear Extracts.
Samples tested
• Immunoprecipitated HAT from cells and tissue • Recombinant enzyme • Nuclear Extract
Test
Biovision supplies other types of Assays as 1.