HDAC3 Inhibitor Screening Kit (Fluorometric)

Catalog number
HDAC3 Inhibitor Screening Kit (Fluorometric)
100 assays
581.00 EUR


Kit's description
Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In BioVision’s HDAC-3 Inhibitor screening Kit, HDAC-3 and developer will deacetylate and cleave the substrate [Arg-His-Lys-Lys(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex=380/500 nm. In presence of a HDAC-3 inhibitor, the cleavage will be inhibited. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC-3 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare the efficacy of the test inhibitors.
• Detection method: Fluorescence (Em/Ex = 380/500 nm) • Applications: Suitable for high throughput screening of HDAC3 inhibitors
Kit's benefits
• Simple procedure; takes ~ 60 min • Fast and convenient • Kit contains the necessary reagents for testing putative HDAC3 inhibitors
Kit's other name
• HDAC3 Assay Buffer • HDAC3 Substrate • HDAC-3 Enzyme • Developer • Trichostatin A
Storage condition
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Samples tested
Testing candidate HDAC3 inhibitors
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.