Cholesterol Detection Kit (cell-based)

Catalog number
K587-100
Name
Cholesterol Detection Kit (cell-based)
Size
100 assays
Price
297.00 EUR
Supplier

Details

Kit's description
Cholesterol is an essential structural component of animal cell membranes. It is required to maintain membrane structural integrity and fluidity. Cholesterol also serves as a precursor for the biosynthesis of several hormones, bile acid and vitamin D. Plasma membrane contains majority of the cellular cholesterol (80-90%) whereas little cholesterol resides in endoplasmic reticulum and in mitochondrial membrane. Transport of intracellular cholesterol within the cells to different compartments is through vesicular and non-vesicular pathways. Defects in these transport processes can alter cellular cholesterol metabolism resulting in pathological conditions. Filipin III is widely used as a probe for sterol localization in membranes. Interaction with cholesterol alters Filipin absorption and fluorescence spectra. This assay provides a simple, easy to perform, histochemical method of identification of unesterified cholesterol.
Highlights
• Detection method: Light and Fluorescence Microscopy (Ex/Em = 340:380/385:470 nm). • Applications: Screen/study/characterize stimulators/inhibitors that affect cholesterol transport and localization in a variety of cells.
Kit's benefits
• Simple, easy to perform, histochemical method of identification of unesterified cholesterol. • Includes U-18666A, a cholesterol transport inhibitor.
Category
Cholesterol
Kit's other name
none
Contents
• Fixative Solution • Assay Buffer • Staining Dye • Inhibitor (U-18666A, 2.5 mM)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Screen/study/characterize stimulators/inhibitors that affect cholesterol transport and localization in a variety of cells.
Samples tested
Cells
Tissue
cell
Description
For cells, cell lines and tissues in culture till half confluency.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.