Galactose and Lactose Colorimetric/Fluorometric Assay Kit

Galactose (C₆H₁₂O₆ FW: 180.16) and lactose (C₁₂H₂₂O₁₁ FW: 342.3) are naturally occurring sugars. Lactose consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage. BioVision’s Galactose / Lactose Assay Kit provides a simple, convenient means for direct measurement of galactose and lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). To detect Galactose, galactose oxidase specifically oxidizes free galactose, generating a product that reacts with the Galactose Probe to produce color (O.D 570nm) and fluorescence (Ex/Em 535/587). To detect lactose, lactose is hydrolyzed by lactase to generate free galactose which is then measured. Thus, Lactose level = Total Galactose – Free Galactose.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian

• Simple procedure; takes ~ 1 hour • Fast and convenient • Kit provides all necessary buffers and reagents for a accurate assay of galactose and lactose individually in various biological samples

Galactose and Lactose Assay

none

• Galactose Assay Buffer • Galactose Probe (DMSO solution) • Lactase (Lyophilized) • Galactose Enzyme Mix (Lyophilized) • HRP (Lyophilized) • Galactose Standard (100nmol/µl)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, monitoring levels during fermentation and feeding in protein expression processes

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.