Heme Colorimetric Assay Kit

Catalog number
K672-100
Name
Heme Colorimetric Assay Kit
Size
100 assays
Price
469.00 EUR
Supplier

Details

Kit's description
Free hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. It can be detected in various body fluids such as saliva, urine and csf under various pathological states. Free hemin exists in cells at a very minute concentrations (<1µM ≈ 650 ng/ml) exerting regulatory functions such as repression of nonspecific δ-aminolevulinate synthase expression and induction of microsomal hemin oxygenase-1. Hemin can stimulate growth of oral bacteria involved in gingivitis and is an indicator of possible pathological conditions when found in the urine or feces. BioVision’s Hemin Assay Kit utilizes peroxidase activity in the presence of hemin to provide a simple, exquisitely sensitive assay which causes the conversion of a colorless probe to a strongly colored (λ = 570) compound. Trace amounts of Hemin can be quantitated in the 5-160 pg (10-250 fmol) range.
Highlights
• Detection method: Absorbance (570 nm) • Species reactivity: Mammalian • Application: Trace amounts of Hemin can be quantitated in the 5:160 pg (10:250 fmol) range.
Kit's benefits
• Simple procedure; takes ~ 30 minutes to complete the assay • Fast and convenient • Highly sensitive assays for measuring Heme level in biological samples
Category
Heme
Kit's other name
none
Contents
• Hemin Assay Buffer • Hemin Probe • DMSO (anhydrous) • Enzyme Mix • Hemin Substrate • Hemin Standard (1 nmol)
Storage condition
4°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth media and food products
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.