Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit

α-Ketoglutarate Dehydrogenase (α-KGDH) (EC 1.2.4.2) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). α-KGDH converts α-ketoglutarate into succinyl-CoA in the presence of NAD and CoA. It is highly regulated by intracellular ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative diseases such as Alzheimer’s disease. Recent studies show that α-KGDH is a target of oxidative stress; reactive oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit. BioVision’s α-KGDH assay kit provides a quick and easy way for monitoring α-KGDH activity in various samples. In the assay, α-KGDH converts α-ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect α-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples.

• Detection method:Absorbance (450 nm) • Applications: : Measurement of α:Ketoglutarate dehydrogenase activity in various tissues/cells : Analysis of cell signaling pathways such as citrate acid cycle, lysine degradation or tryptophan metabolism in various cell types

• Simple, rapid & convenient • can measure α-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples

alpha-Ketoglutarate Dehydrogenase

none

• KGDH Assay Buffer • KGDH Substrate (Lyophilized) • KGDH Developer (Lyophilized) • NADH Standard (Lyophilized) • KGDH Positive Control

-20°C

gel pack

12 months

see datasheet

• Measurement of α-Ketoglutarate dehydrogenase activity in various tissues/cells • Analysis of cell signaling pathways such as citrate acid cycle, lysine degradation or tryptophan metabolism in various cell types

• Animal tissues: liver, heart, muscle, etc. • Purified mitochondria • Cell culture: Adherent or suspension cells

The Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.