Inosine Fluorometric Assay Kit

Inosine is commonly found in tRNAs and is essential for proper translation of the genetic code in wobble base pairs. Knowledge of inosine metabolism has led to advances in immunotherapy in recent decades. Inosine and hypoxanthine are also potential markers of ATP catabolic-by-products from oxidative stress which follows acute cardiac ischemia. In BioVision’s Inosine Assay Kit, inosine is converted to hypoxanthine, which will react with the substrate mix and PicoProbe™ to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes. This assay has a detection limit of of approximately 100 pmol inosine/well.

• Detection method: Fluorescence (Ex/Em = 535/587 nm) • Species reactivity: Mammalian • Application: This assay has a detection limit of of approximately 100 pmol inosine/well.

• Simple procedure; takes ~ less than 40 minutes • Fast and convenient • Sensitive assays for measuring Inosine levels in a variety of samples.

Inosine

none

• Inosine Assay Buffer • PicoProbe™ • Converter Enzyme • Developer Enzyme • Inosine Substrate Mix • Inosine Standard (10 mM)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, etc.

Biovision supplies other types of Assays as 1.