Myeloperoxidase (MPO) Inhibitor Screening Kit (Fluorometric)

Catalog number
Myeloperoxidase (MPO) Inhibitor Screening Kit (Fluorometric)
200 assays
732.00 EUR


Kit's description
Myeloperoxidase (MPO: EC is a peroxidase abundantly expressed in neutrophil granulocytes. It catalyzes the hydrogen peroxide dependent oxidation of halide ions to generate hypochlorite (HClO), the reaction by which MPO exhibits cytotoxic activity against tumor cells and microorganisms. MPO also oxidizes various substances such as phenol and anilines. MPO undergoes chlorination or peroxidation reaction depending upon the relative concentrations of chloride and the reducing substrates. Recent studies suggest that increased levels of MPO are associated with an increased risk for cardiovascular disease and myocardial infarction, thus development of novel and specific inhibitors of MPO is critical for therapeutic purposes. BioVision’s MPO Inhibitor Screening Kit provides screening assays for both MPO chlorination and peroxidation activities. In the chlorination assay (specific for MPO), MPO converts hydrogen peroxide and sodium chloride to sodium hypochlorite, which reacts with Chlorination substrate to give an intensely fluorescent product (Ex/Em = 480/520 nm). In the Peroxidation Assay, MPO oxidizes peroxidation substrate to generate fluorescence (Ex/Em = 535/587 nm). The fluorescence generated is directly proportional to any peroxidase activity present. In the presence of MPO inhibitor, reactions are impeded, thus decreasing the rate and/or extent of generation of MPO-dependent fluorescence. This kit provides a sensitive, quick, and easy method for screening potential inhibitors of MPO, and identifying whether one or both activities are inhibited. MPO Inhibitor Control is included to compare the efficacy of test inhibitors. The assay is high-throughput adaptable and can be performed in less than 20 min.
• Detection method: Fluorescence (Ex/Em 535/587 nm) • Applications: Screening/characterizing/studying potential inhibitors of MPO
Kit's benefits
• Rapid and simple • Reliable test for screening potential inhibitors of MPO • Includes Inhibitor Control [4-Aminobenzonic Hydrazide]
MPO, Myeloperoxidase, Myeloperoxidase inhibitor, MPO inhibitor
Kit's other name
• MPO Assay Buffer • MPO Chlorination Substrate (in DMSO) • MPO Peroxidation Substrate (in DMSO) • MPO Enzyme • Inhibitor Control (4-Aminobenzonic Hydrazide, in DMSO) • Hydrogen Peroxide
Storage condition
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Screening/characterizing/studying potential inhibitors of MPO
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.