Glutathione Peroxidase Activity Colorimetric Assay Kit

Catalog number
Glutathione Peroxidase Activity Colorimetric Assay Kit
100 assays
480.00 EUR


Kit's description
Glutathione Peroxidase (GPx, EC is an enzyme family with peroxidase activity, and plays important role in protecting of organisms from oxidative damage. It converts reduced glutathione (GSH) to oxidized glutathione (GSSG), to reduce lipid hydroperoxides to their corresponding alcohols, or reduce free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders. BioVision’s Glutathione Peroxidase Assay Kit measures glutathione peroxidase (GPx) activity through a coupled reaction with glutathione reductase (GR). In the assay, GPx reduce Cumene Hydroperoxide, and oxidize GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by GR. The decrease of NADPH is proportionally to GPx activity in the reactions. The decrease of NADPH can be easily measured by absorbance at 340 nm. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with detection sensitivity ~ 0.5 mU/ml of GPx in samples.
• Detection method: Absorbance (340 nm) • Species reactivity: Mammalian • Application: The assay has a detection sensitivity ~ 0.5 mU/ml of GPx in samples.
Kit's benefits
• Simple procedure; takes ~ 30-40 minutes • Fast and convenient
Glutathione reductase
Kit's other name
• GPx Assay Buffer • NADPH (lyophilized) • Glutathione Reductase • Glutathione (GSH, lyophilized) • Cumene Hydroperoxide • GPx Positive Control (lyophilized)
Storage condition
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Samples tested
Cell and tissue culture supernatants, urine, plasma and serum, erythrocyte lysates, as well as many other biological fluids, etc.
Biovision supplies other types of Assays as 1.
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH