Beta Galactosidase (β-Gal) Activity Assay Kit (Fluorometric)

Beta Galactosidase (β-Gal, EC: 3.2.1.23) is an enzyme which hydrolyzes the β-galactosides into monosacchanides. β-Gal is widely used as a reporter gene in the field of molecular biology. Senescence Associated β-Gal (SA-β-Gal) is an isoform of β-Gal which has the optimal activity at pH 6.0, and is mostly used as a biomarker for senescent cells (K802). β-Gal is an essential enzyme in humans and its deficiency results in Morquio’s Syndrome, a severe birth defect. BioVision’s Beta Galactosidase Activity Assay kit provides a quick and easy way for monitoring Beta Galactosidase activity in a variety of samples. In this kit, Beta Galactosidase hydrolyses a non-fluorescent substrate to generate a strong fluorescent product. The assay is simple, sensitive, and high-throughput adaptable. Detection limit: < 0.1 mU.

• Detection method: Fluorescence (Ex/Em = 480/520 nm) • Application: Measurement of β:Galactosidase activity in various samples

• Quick and easy method to measure Beta Galactosidase activity. • Detection limit: < 0.1 mU.

Beta Galactosidase

none

• β-Gal Assay Buffer • β-Gal Substrate (in DMSO) • Fluorescein Standard (1 mM) • β-Gal Positive Control

-20°C

gel pack

12 months

see datasheet

• Measurement of β-Galactosidase activity in various samples

• Prokaryote such as: E.coli • Animal tissues such as spleen etc. • Adherent or suspension cells • Food such as yogurt • White blood cells

Biovision supplies other types of Assays as 1.