Deproteinizing Sample Preparation Kit II

The study of biofluids, cell, bacteria, yeast, and tissue lysates has delivered vital information regarding their biochemical composition. These biological samples comprise a rich, complex list of lipids, proteins, small organic molecules, ions, and, depending on the sample, exogenous molecules (i.e. drugs). Several small organic molecules can also be found free and/or bound to other macromolecules. These macromolecules (lipoproteins, proteins, enzymes) interfere or degrade low molecular-weight metabolites. Therefore, sample deproteinization is often required. Chemical deproteinization procedure such as Trichloroacetic acid (TCA) is one of the most utilized methods to accomplish sample deproteinization. BioVision’s Deproteinizing Sample Preparation Kit II offers an excellent alternative when metabolite lability affects the accurate detection and estimation of small molecule concentrations and when organic solvents or ultrafiltration cannot be used. TCA precipitates proteins by lowering the sample pH drastically. After removal of precipitated proteins, the pH of the sample is neutralized with neutralization buffer that is provided in the kit. The kit is easy to follow, convenient, and can be utilized for the preparation of a large number of samples in parallel. Samples prepared using this kit can be directly used in various bioassays.

Unique tool to prepare samples for the analysis of a variety of small molecules. Proteins are precipitated without using perchloric acid (PCA)

• Fast and convenient • Samples prepared using this kit can be directly used in a wide variety of small molecule bioassays.

Protein removal

none

• TCA • Neutralization Solution

RT

RT

12 months

see datasheet

Inquire

• Biofluids: serum, plasma, whole blood, saliva, urine etc. • Lysates: cell culture, mammalian tissue, yeast, bacteria etc.