EZLabel™ Protein Biotin Labeling Kit

BioVision’s EZLabel™ Protein-Biotin Labeling Kit provides an easy way to label proteins with Biotin in a user-friendly spin column format. Due to its high affinity for tetrameric avidin proteins, biotin is ideal for labeling proteins. Each pair of spin columns provided in the kit can be used to purify up to 1 mg of the labeled target protein. The kit provides all of the reagents necessary to perform five labeling reactions using up to 1 mg of protein per reaction. Biotin-labeled protein can be directly used for multiple downstream applications including ELISA, western blot, Immunohistochemistry, Immunoassays, etc.

Easy to use Kit to label upto 5 mg of Protein with Biotin for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis

All necessary reagents included. Fast and efficient labeling of proteins with Biotin

Protein labeling

none

EZLabel™ Biotin EZLabel™ Spin Column EZLabel™ Elution Buffer

+4°C

gel pack

12 months

broad

Labeled proteins can be used for ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis

Proteins

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Biotinylated