Granzyme B (human) ELISA Kit

Catalog number
Granzyme B (human) ELISA Kit
100 assays
834.00 EUR


Kit's description
Granzyme B is a serine protease expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells and functions in cell-mediated immunity. BioVision’s human Granzyme B ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human Granzyme B is coated on a 96-well plate. Standards and test samples are added to the wells and Granzyme B present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for Granzyme B is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Granzyme B captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 10 pg/ml.
• Detection method: Absorbance (450 nm) • Species reactivity:mouse • Application: Quantitative protein detection, establishing normal range, validation of antibody array results
Kit's benefits
• Easy, convenient and time-saving method to assay for human Granzume B. • Detection Range:15.6 pg/ml – 1000 pg/ml. Sensitivity: < 10 pg/ml. • This ELISA kit shows no cross-reactivity with other relevant proteins.
Granzyme B ELISA kit
Kit's other name
Granzyme B
• 96 wells coated with anti-human monoclonal Granzyme B antibody, 1 Microplate • Lyophilized recombinant human Granzyme B standard (10 ng/vial) • Biotinylated anti- human Granzyme B antibody • Avidin-Biotin-Peroxidase Complex (ABC) • Sample diluent buffer • Antibody diluent buffer • ABC diluent buffer • TMB color developing agent (Colorless) • TMB stop solution
Storage condition
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Application- Quantitative protein detection, establishing normal range
Samples tested
• Serum & plasma (heparin, EDTA) • Cell culture supernatants
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED