Beta-NGF (human) ELISA Kit
Catalog number
K4787-100
Name
Beta-NGF (human) ELISA Kit
Size
100 assays
Price
909.00 EUR
Supplier
Details
Kit's description
BioVision’s Human Beta-NGF (beta-nerve growth factor) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Beta-NGF. This assay employs an antibody specific for human Beta-NGF coated on a 96-well plate. Standards and samples are pipetted into the wells and Beta-NGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Beta-NGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Beta-NGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of Beta-NGF is typically less than 14 pg/ml. Detection Range: 14 pg/ml – 5,000 pg/ml. The intra-Assay reproducibility is CV
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results
Kit's benefits
• Easy, convenient and time-saving method to assay Beta-NGF. • The minimum detectable concentration of Beta-NGF is less than 14 pg/ml. •This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.
Category
Beta-NGF
Kit's other name
Beta-Nerve Growth Factor (human) ELISA Kit
Contents
• Beta-NGF Ab coated plate (Item A), 96 wells • Wash Buffer Concentrate (20x) (Item B) • Human Beta-NGF Standard (Item C) • Assay Diluent (5x) (Item E) • Biotinylated anti-human Beta-NGF Ab (Item F) • HRP-Streptavidin Concentrate (800x) (Item G) • TMB (Item H) • Stop Solution (Item I)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
human
Applications
Quantitative protein detection, establishing normal range, validation of antibody array results
Samples tested
• Serum & plasma (collect plasma using heparin as an anticoagulant. EDTA & citrate are not recommended. • Cell culture supernatants • Urine
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED