Vitamin D3 (human) ELISA kit

Catalog number
Vitamin D3 (human) ELISA kit
100 assays
642.00 EUR


Kit's description
Vitamin D3 (Cholecalciferol) is found in human epidermis and dermis. BioVision’s human Vit. D3 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human Vit. D3 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Vit. D3 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Vit. D3 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 20 – 800 µg/L.
• Detection method: Absorbance (450 nm) • Species reactivity:human • Application: Quantitative protein detection, establishing normal range.
Kit's benefits
• Easy, convenient and time-saving method to assay for human vitamin D3. • Detection Range:200 – 800 ug/L. • This ELISA kit shows no species cross-reactivity.
Vitamin D3 ELISA kit
Kit's other name
Human Vitamin D3 (Cholecalciferol) ELISA kit
• 96 wells coated with anti-human Vit. D3 antibody, 1 Microplate • Human Vit. D3 standard (0.9 ng/µl) • HRP-conjugate reagent • Standard Diluent • Sample diluent • Chromogen Solution A • Chromogen Solution B • Stop Solution • Wash Solution (30x stock) • Sealed Bag • Plate cover
Storage condition
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Quantitative protein detection, establishing normal range, validation of antibody array results
Samples tested
• Serum & plasma (EDTA/Citrate) • Urine • Cell culture medium, Tissue homogenates and cell lysates
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED