(15-biotinlamino-4,7,10,13-dioxanonanoic acid N-hydroxysuccinimidyl ester)
White waxy solid
≥97% by HPLC
H2₂O (10 mg/ml) or DMF (10 mg/ml)
Protect from air and moisture
A water-soluble, pegylated reagent for simple and efficient biotin labeling of antibodies, proteins and other primary-amine containing molecules.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.