Human CellExp UPA, human recombinant

Catalog number

7248-10

Size

10 μg

Price

395.00 EUR

Supplier

Biovision

Order

Details

Synonyms

Urokinase, PLAU, ATF, UPA, URK, u-PA，BDPLT5，QPD

Alternates names

Urokinase, PLAU, ATF, UPA, URK, u-PA，BDPLT5，QPD

Taglines

A potent marker of invasion and metastasis in a variety of human cancers

Biomolecule/Target

UPA

NCBI Gene ID #.

5328

NCBI Gene Symbol

uPA

Gene Source

Human

Accession #

P00749

Recombinant

Yes

Source

HEK 293 cells

Purity

N/A

Endotoxin Level

< 0.1 ng/μg of protein (

Activity (Specifications/test method)

Measured by its ability to cleave a peptide substrate, N - carbobenzyloxy - Gly - Gly - Arg - 7 - amido - 4 - methylcoumarin (Z - GGR - AMC). The specific activity is > 2000 pmoles/min/μg.

Biological activity

Measured by its ability to cleave a peptide substrate, N - carbobenzyloxy - Gly - Gly - Arg - 7 - amido - 4 - methylcoumarin (Z - GGR - AMC). The specific activity is > 2000 pmoles/min/μg.

Results

N/A

Molecular Weight

This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 45 kDa. The predicted N-terminus is Ser21, Ile179 & Lys156 . DTT-reduced Protein migrates as three bands corresponding to the long α chain, β chain and unprocessed full-length chain with the molecular mass of 18 kDa, 32 kDa and 50 kDa respectively due to glycosylation and cleavage.

Storage Temp.

-20°C

Shipping

Gel Pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized powder

Physical form description

Lyophilized from 0.22 μm filtered solution in HEPES, NaCl and CaCl2. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants