Human CellExp UPA, human recombinant

Catalog number
7248-50
Name
Human CellExp UPA, human recombinant
Size
50 μg
Price
1070.00 EUR
Supplier

Details

Synonyms
Urokinase, PLAU, ATF, UPA, URK, u-PA,BDPLT5,QPD
Alternates names
Urokinase, PLAU, ATF, UPA, URK, u-PA,BDPLT5,QPD
Taglines
A potent marker of invasion and metastasis in a variety of human cancers
Biomolecule/Target
UPA
NCBI Gene ID #.
5328
NCBI Gene Symbol
uPA
Gene Source
Human
Accession #
P00749
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
Measured by its ability to cleave a peptide substrate, N - carbobenzyloxy - Gly - Gly - Arg - 7 - amido - 4 - methylcoumarin (Z - GGR - AMC). The specific activity is > 2000 pmoles/min/μg.
Biological activity
Measured by its ability to cleave a peptide substrate, N - carbobenzyloxy - Gly - Gly - Arg - 7 - amido - 4 - methylcoumarin (Z - GGR - AMC). The specific activity is > 2000 pmoles/min/μg.
Results
N/A
Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 45 kDa. The predicted N-terminus is Ser21, Ile179 & Lys156 . DTT-reduced Protein migrates as three bands corresponding to the long α chain, β chain and unprocessed full-length chain with the molecular mass of 18 kDa, 32 kDa and 50 kDa respectively due to glycosylation and cleavage.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Lyophilized from 0.22 μm filtered solution in HEPES, NaCl and CaCl2. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants