Human CellExp VEGF164, mouse recombinant

Catalog number

7425-10

Size

10 μg

Price

455.00 EUR

Supplier

Biovision

Order

Details

Synonyms

VEGF164, VEGFA, VPF

Alternates names

VEGF164, VEGFA, VPF

Taglines

Biomolecule/Target

VEGF164

NCBI Gene ID #.

22339

NCBI Gene Symbol

VEGFA

Gene Source

Mouse

Accession #

Q00731

Recombinant

Yes

Source

HEK293 cells

Purity

N/A

Activity (Specifications/test method)

N/A

Biological activity

Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 3.5 ng/ml.

Results

Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 3.5 ng/ml.

Molecular Weight

Mouse VEGF164 contains no "tag", and has a calculated MW of 19.3 kDa. The predicted N-terminus is Ala 27. DTT-reduced Protein migrates as 23-25 kDa in SDS-PAGE due to different glycosylation.

Storage Temp.

-20°C

Shipping

Gel Pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized

Physical form description

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus

Additional source

Recombinants or rec. proteins

Group

recombinants