Human CellExp CD62E/E-Selectin, human recombinant

Catalog number
7434-20
Name
Human CellExp CD62E/E-Selectin, human recombinant
Size
20 μg
Price
355.00 EUR
Supplier

Details

Synonyms
SELE, RP1-117P20.2, CD62E, ELAM1, ESEL, LECAM2, E-Selectin
Alternates names
SELE, RP1-117P20.2, CD62E, ELAM1, ESEL, LECAM2, E-Selectin
Taglines
Plays an important part in recruiting leukocytes to the site of injury.
Biomolecule/Target
CD62E/E-Selectin
NCBI Gene ID #.
6401
NCBI Gene Symbol
SELE
Gene Source
Human
Accession #
P16581
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10^4 cells/well are added to human E-Selectin coated plates (2 μg/mL with 100 μL/well), >80% cells will adhere after 1 hour incubation at 37°C.
Results
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10^4 cells/well are added to human E-Selectin coated plates (2 μg/mL with 100 μL/well), >80% cells will adhere after 1 hour incubation at 37°C.
Molecular Weight
This protein is fused with a 6×his tag at C-terminus and has a calculated MW of 60 kDa expressed. The predicted N-terminus is Trp22. Protein migrates as 110 kDa in reduced SDS-PAGE resulting from glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants