Human CellExp PD-L1 /CD274 /B7-H1, human recombinant

Catalog number
7429-250
Name
Human CellExp PD-L1 /CD274 /B7-H1, human recombinant
Size
250 μg
Price
1995.00 EUR
Supplier

Details

Synonyms
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Alternates names
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Taglines
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
Biomolecule/Target
PD-L1 /CD274 /B7-H1
NCBI Gene ID #.
29126
NCBI Gene Symbol
CD274
Gene Source
Human
Accession #
Q9NZQ7
Recombinant
Yes
Source
HEK293 cells
Purity
?90%
Endotoxin Level
N/A
Activity (Specifications/test method)
Measured by its binding ability in a functional ELISA
Biological activity
Immobilized Human PD-L1, His Tag at 5 ?g/mL (100 ?L/well) can bind Human PD-1, His Tag with a linear range of 0.15-1.25 ?g/mL.
Results
N/A
Molecular Weight
This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 26.4 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 35-40 kDa due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 ?m filtered solution in PBS pH 7.4
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants